Glutathione S-transferases prediction of functional subfamilies protein design and enzyme engineering protein structure–function relationship subfamily-specific positions.Menus Icon Bar Menu Icon Accordion Tabs Vertical Tabs Tab Headers Full Page Tabs Hover Tabs Top Navigation Responsive Topnav Split Navigation Navbar with Icons Search Menu Search Bar Fixed Sidebar Side Navigation Responsive Sidebar Fullscreen Navigation Off-Canvas Menu Hover Sidenav Buttons Sidebar with Icons Horizontal Scroll Menu Vertical Menu Bottom Navigation Responsive Bottom Nav Bottom Border Nav Links Right Aligned Menu Links Centered Menu Link Equal Width Menu Links Fixed Menu Slide Down Bar on Scroll Hide Navbar on Scroll Shrink Navbar on Scroll Sticky Navbar Navbar on Image Hover Dropdowns Click Dropdowns Cascading Dropdown Dropdown in Topnav Dropdown in Sidenav Resp Navbar Dropdown Subnavigation Menu Dropup Mega Menu Mobile Menu Curtain Menu Collapsed Sidebar Collapsed Sidepanel Pagination Breadcrumbs Button Group Vertical Button Group Sticky Social Bar Pill Navigation Responsive Header Zebra results are provided in two ways - (1) as a single all-in-one parsable text file and (2) as PyMol sessions with structural representation of SSPs. ![]() ![]() ![]() Statistical analysis is implemented to automatically select a set of highly significant SSPs to be used as hotspots for directed evolution or rational design experiments and analyzed studying the structure-function relationship. It is the first application that provides specificity determinants at different levels of functional classification, therefore addressing complex functional diversity of large superfamilies. This paper describes Zebra web server - a powerful remote platform that implements a novel bioinformatic analysis algorithm to study diverse protein families. Consequently, a corresponding method to perform the bioinformatic analysis of sequence and structural data has to be implemented in the common laboratory practice to study the structure-function relationship in proteins and develop novel protein engineering strategies. The subfamily-specific positions (SSPs) are conserved within functional subfamilies but are different between them and, therefore, seem to be responsible for functional diversity in protein superfamilies. ![]() A systematic study of the corresponding adaptive mutations provides a key to one of the most challenging problems of modern structural biology - understanding the impact of amino acid substitutions on protein function. During evolution of proteins from a common ancestor, one functional property can be preserved while others can vary leading to functional diversity.
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